| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
El Ghissassi, Awab (10)
|
NIVEST |
39.08 |
37.86 |
-1.22 |
| 2 |
Lind, Austin (10)
|
NICORY |
40.47 |
40.01 |
-0.46 |
| 3 |
McGovern, Colin M (9)
|
NIUNAC |
45.26 |
44.12 |
-1.14 |
| 4 |
Yu, Michael (10)
|
NIKRKN |
47.46 |
44.23 |
-3.23 |
| 5 |
Law, Linus C (10)
|
NIKRKN |
54.75 |
45.44 |
-9.31 |
| 6 |
Fritz, Will S (10)
|
NIISWM |
|
45.63 |
- |
| 7 |
Worob, Jack H (9)
|
NIISWM |
45.08 |
46.67 |
1.59 |
| 8 |
Gorman, Ryan P (10)
|
NIWAAC |
46.69 |
46.70 |
0.01 |
| 9 |
Mangoba, Zachary A (9)
|
NIISWM |
47.41 |
46.71 |
-0.70 |
| 10 |
Benton, Jack D (10)
|
NIKRKN |
52.92 |
47.05 |
-5.87 |
| 11 |
Figueroa, John L (9)
|
NICAGO |
1:03.07 |
47.15 |
-15.92 |
| 12 |
Hicks, Ethan M (10)
|
NIWAAC |
53.92 |
49.13 |
-4.79 |
| 13 |
Losinger, Shawn B (9)
|
NIUNAC |
52.05 |
50.72 |
-1.33 |
| 14 |
Urazgildiiev, Timur (7)
|
NIISWM |
52.26 |
50.93 |
-1.33 |
| 15 |
Venkataswamy, Charan (9)
|
NIWAAC |
1:05.04 |
51.05 |
-13.99 |
| 16 |
Quintana, Cyrus A (10)
|
NIKVAC |
|
51.41 |
- |
| 17 |
Leonard, Joshua D (10)
|
NIWAAC |
49.37 |
52.29 |
2.92 |
| 18 |
Parker, William A (8)
|
NIKRKN |
1:02.55 |
53.27 |
-9.28 |
| 19 |
Clark, Aidan J (9)
|
NIWAAC |
52.41 |
53.76 |
1.35 |
| 20 |
Ankam, Ronel P (9)
|
NIWAAC |
52.59 |
54.16 |
1.57 |
| 21 |
Anderson, Koen T (9)
|
NIISWM |
53.27 |
54.46 |
1.19 |
| 22 |
Jusianiec, Jackson M (10)
|
NIKVAC |
1:04.78 |
55.90 |
-8.88 |
| 23 |
Nale, Joshua D (9)
|
NIUNAC |
1:02.43 |
58.34 |
-4.09 |
| 24 |
Hollandt, Anderson E (10)
|
NIUNAC |
59.30 |
58.40 |
-0.90 |
| 25 |
Lawrence, Henry J (9)
|
NIKVAC |
|
1:01.74 |
- |
| 26 |
Hwang, Nicholas C (9)
|
NIISWM |
|
1:01.87 |
- |
| 27 |
Whittaker, Mekhi A (9)
|
NIUNAC |
|
1:02.50 |
- |
| 28 |
Carbone, Nicholas L (10)
|
NIGGST |
1:08.02 |
1:06.50 |
-1.52 |
| 29 |
Law, Cyrus C (7)
|
NIKRKN |
1:10.21 |
1:09.21 |
-1.00 |
| 30 |
Sattur, Abhinav R (8)
|
NIWAAC |
|
1:11.19 |
- |
| 31 |
Ankam, Shayan T (6)
|
NIWAAC |
|
1:28.99 |
- |