| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Chaplin, Allison V (14)
|
NITTSC |
28.81 |
29.21 |
0.40 |
| 2 |
Clouser, Kristiana I (13)
|
NIBCSR |
28.83 |
29.62 |
0.79 |
| 3 |
Drews, Maren G (13)
|
NITTSC |
28.82 |
29.64 |
0.82 |
| 4 |
Donlon, Anna M (13)
|
NIWWSC |
30.11 |
30.08 |
-0.03 |
| 5 |
Avila-Shah, Jessi E (14)
|
NIWWSC |
30.38 |
30.09 |
-0.29 |
| 6 |
Sousis, Gabriella R (14)
|
NISTAR |
29.43 |
30.20 |
0.77 |
| 7 |
Higley, Kayla P (14)
|
NIRAYS |
30.69 |
30.99 |
0.30 |
| 8 |
Calderon, Jennifer A (14)
|
NISTAR |
|
31.76 |
- |
| 9 |
Hauser-Howells, Lillian K (13)
|
NITTSC |
33.91 |
34.03 |
0.12 |
| 10 |
Zaccarine, Brooke D (13)
|
NIRAYS |
34.20 |
34.81 |
0.61 |
| 11 |
Polek, Sarah G (13)
|
NITTSC |
34.48 |
35.51 |
1.03 |
| 12 |
Cameron, Nzingha A (13)
|
NIBCSR |
35.49 |
36.54 |
1.05 |