| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
El Ghissassi, Sidra S (9)
|
NIVEST |
43.34 |
41.21 |
-2.13 |
| 2 |
Owczarzak, Madeline L (10)
|
NICSCI |
43.30 |
41.98 |
-1.32 |
| 3 |
Ryan, Natalie C (10)
|
NIUNAC |
45.13 |
43.42 |
-1.71 |
| 4 |
Devendorf, Madelyn G (10)
|
NIJETS |
45.46 |
43.84 |
-1.62 |
| 5 |
Stewart, Katelyn J (10)
|
NIJETS |
44.69 |
44.53 |
-0.16 |
| 6 |
Gucciardi, Molly S (9)
|
NIJETS |
43.74 |
44.57 |
0.83 |
| 7 |
Ioannisci, Gianna M (9)
|
NIUNAC |
42.89 |
44.69 |
1.80 |
| 8 |
Omans, Elaina M (9)
|
NISKAN |
46.71 |
44.76 |
-1.95 |
| 9 |
Schrader, Olivia Q (10)
|
NIUNAC |
45.71 |
45.96 |
0.25 |
| 10 |
Snell, Kaitlyn A (10)
|
NINAYE |
49.41 |
48.23 |
-1.18 |
| 11 |
Geraci, Taylor M (9)
|
NIOLSC |
|
49.32 |
- |
| 12 |
Hann, Rachel S (10)
|
NICSCI |
50.35 |
49.33 |
-1.02 |
| 13 |
Johnson, Mia (9)
|
NISKAN |
|
49.39 |
- |
| 14 |
Vallone, Francesca G (10)
|
NIVEST |
50.28 |
50.24 |
-0.04 |
| 15 |
Armijo, Julia A (10)
|
NISKAN |
57.15 |
51.48 |
-5.67 |
| 16 |
Wiechmann, Olivia (9)
|
NIJETS |
56.62 |
51.60 |
-5.02 |
| 17 |
Pappa, Alexis J (10)
|
NIOLSC |
55.55 |
51.81 |
-3.74 |
| 18 |
Chandler, Nora M (9)
|
NIUN-N |
|
52.17 |
- |
| 19 |
Fleckenstein, Lucy (10)
|
NISKAN |
|
53.45 |
- |
| 20 |
Gerges, Christina F (10)
|
NIJETS |
58.33 |
53.80 |
-4.53 |
| 21 |
Reynolds, Hailey A (10)
|
NINAYE |
1:00.91 |
54.23 |
-6.68 |
| 22 |
Davis, Ella J (10)
|
NIOLSC |
|
54.67 |
- |
| 23 |
Andrianos, Alivia K (10)
|
NIJETS |
1:03.55 |
55.68 |
-7.87 |
| 24 |
Saucier, Ireland (9)
|
NISKAN |
|
1:03.60 |
- |
| 25 |
Wojieck, Teagan E (8)
|
NIWGSC |
1:01.13 |
1:05.27 |
4.14 |
| 26 |
Smith, Myah J (10)
|
NIMETS |
|
1:13.11 |
- |
| 27 |
Clark, Isabel J (7)
|
NIMETS |
|
1:19.09 |
- |
| 28 |
Youngs, Madelyn (10)
|
NISKAN |
|
1:30.01 |
- |
|
Kadylak, Liana J (8)
|
NIUNAC |
|
DQ |
- |
|
Jennings, Amelia R (10)
|
NICSCI |
55.88 |
DQ |
- |
|
Buckley, Elizabeth G (9)
|
NICSCI |
|
DQ |
- |