| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kabunov, Mikhail V (11)
|
NIJETS |
32.86 |
31.56 |
-1.30 |
| 2 |
Peters, Evan M (12)
|
NIGCOM |
35.99 |
34.51 |
-1.48 |
| 3 |
Mills, William P (12)
|
NIMETS |
35.24 |
35.17 |
-0.07 |
| 4 |
Caswell, Christopher R (12)
|
NIGCOM |
37.33 |
35.74 |
-1.59 |
| 5 |
VanAlst, Amon J (11)
|
ADUNAT |
41.13 |
39.11 |
-2.02 |
| 6 |
Monroe, Lucas J (12)
|
NIPMSC |
42.58 |
39.75 |
-2.83 |
| 7 |
Anthony, Jaydon T (12)
|
NIPMSC |
41.37 |
40.90 |
-0.47 |
| 8 |
Britz, Malcolm T (12)
|
NISYCH |
42.12 |
41.06 |
-1.06 |
| 9 |
Lange, Matthew (12)
|
NISKAN |
42.04 |
42.39 |
0.35 |
| 10 |
Milliman, Kaden M (11)
|
NIWGSC |
38.54 |
43.02 |
4.48 |
| 11 |
Kligerman, Eli W (11)
|
NISYCH |
44.94 |
43.73 |
-1.21 |
| 12 |
Finnerty, Sean P (12)
|
NICSCI |
48.28 |
45.61 |
-2.67 |
| 13 |
Williams, Mason F (11)
|
NIUNAC |
43.15 |
45.94 |
2.79 |
| 14 |
Cappellett, Harrison J (11)
|
NIUNAC |
47.80 |
46.51 |
-1.29 |
| 15 |
St John, Treygan J (12)
|
NINAYE |
57.62 |
47.13 |
-10.49 |
| 16 |
Pelin, Tyler H (12)
|
NINAYE |
55.32 |
51.44 |
-3.88 |
| 17 |
Potter, Logan C (11)
|
NIGRAT |
52.17 |
55.81 |
3.64 |
| 18 |
Perry, Kaden J (11)
|
NIMETS |
1:09.13 |
1:05.37 |
-3.76 |