| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Xu, Ming Na (12)
|
NIFAST |
2:24.75 |
2:23.36 |
-1.39 |
| 2 |
Gorton, Claire P (12)
|
NIWEBS |
3:00.00 |
2:46.06 |
-13.94 |
| 3 |
Robinson, Sara C (11)
|
NISDSC |
2:55.09 |
2:50.44 |
-4.65 |
| 4 |
Skizim, Elena K (12)
|
NIWEBS |
2:58.93 |
2:50.61 |
-8.32 |
| 5 |
Trapp, Angelina L (12)
|
NIPMSC |
3:11.12 |
2:56.34 |
-14.78 |
| 6 |
Callaghan, Kelsey J (12)
|
NIVEST |
3:02.03 |
2:58.89 |
-3.14 |
| 7 |
Smolinski, Evelynn J (11)
|
NIHAST |
3:09.87 |
3:05.80 |
-4.07 |
| 8 |
Hagen, Nicolette E (12)
|
NIFAST |
|
3:17.26 |
- |
| 9 |
Resch, Rebecka J (11)
|
NIGRSC |
3:12.84 |
3:29.96 |
17.12 |
| 10 |
Buck, Kathryn E (12)
|
NIWEBS |
|
3:40.70 |
- |
| 11 |
Thurston, Lauren A (11)
|
NIGCOM |
3:44.32 |
3:48.56 |
4.24 |
|
Hubbard, Mary Elena P (12)
|
NIGRSC |
3:53.20 |
NS |
- |
|
VanHouten, Lila S (12)
|
NIPACK |
2:52.63 |
NS |
- |