| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Donato, Timothy P (15)
|
NIFAST |
24.70 |
24.10 |
-0.60 |
| 2 |
Maclaughlin, Daniel (14)
|
NIPACK |
25.36 |
25.20 |
-0.16 |
| 3 |
Riester, Ethan F (15)
|
NISDSC |
26.44 |
25.24 |
-1.20 |
| 4 |
Murray, Maxwell P (15)
|
NISDSC |
25.72 |
25.32 |
-0.40 |
| 5 |
Novozhenets, Benjamin D (13)
|
NIPACK |
26.03 |
26.12 |
0.09 |
| 6 |
Welch, Shane A (15)
|
NIQUIK |
29.30 |
26.22 |
-3.08 |
| 7 |
Ott, David C (13)
|
NIFAST |
26.96 |
26.55 |
-0.41 |
| 8 |
Nenadic, Alexander B (14)
|
NIFAST |
27.79 |
26.57 |
-1.22 |
| 9 |
Gilbert, Ajani V (14)
|
NISDSC |
27.39 |
27.24 |
-0.15 |
| 10 |
Szulis, John W (14)
|
NINEWW |
29.47 |
28.75 |
-0.72 |
| 11 |
Weiner, Lucas M (13)
|
NIFAST |
32.43 |
28.80 |
-3.63 |
| 12 |
Ciravola, Jayden T (15)
|
NIHAST |
29.70 |
29.66 |
-0.04 |
| 13 |
Dane, Samuel L (13)
|
NIVICT |
30.36 |
30.19 |
-0.17 |
| 14 |
Davis, Joshua A (14)
|
NIFAST |
33.40 |
31.66 |
-1.74 |
| 15 |
Pompeii, Joey J (14)
|
NIPMSC |
35.52 |
34.14 |
-1.38 |
|
Green, William E (13)
|
NIHAST |
34.54 |
NS |
- |
|
Bronson, Dylan T (16)
|
NISDSC |
25.82 |
NS |
- |
|
Weiner, Nicholas J (13)
|
NIFAST |
35.40 |
NS |
- |
|
Webb, Douglas J (14)
|
NIFAST |
28.20 |
NS |
- |