| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bush, Katherine E (16)
|
LARRAC |
24.83 |
26.16 |
1.33 |
| 2 |
Krzywanski, Melissa G (16)
|
LARRAC |
27.63 |
27.98 |
0.35 |
| 3 |
Williamson, Clara (15)
|
NTRWY |
28.50 |
28.48 |
-0.02 |
| 4 |
Jones, Haylie (16)
|
NTRWY |
28.28 |
28.57 |
0.29 |
| 5 |
Perry, Kirsten A (16)
|
LARRAC |
29.21 |
29.26 |
0.05 |
| 6 |
Holguin, Annelise (15)
|
COPY |
31.56 |
31.50 |
-0.06 |
| 7 |
Kairschner, Kathryn E (16)
|
LARRAC |
24.74 |
31.89 |
7.15 |
| 8 |
Mathis, Hope N (18)
|
LARRAC |
33.06 |
32.29 |
-0.77 |
| 9 |
Perry, Katherine G (16)
|
LARRAC |
31.82 |
32.41 |
0.59 |
|
Langdon, Mary Belle (15)
|
TNY |
31.55 |
NS |
- |