| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Roberts, Grace E (16)
|
ADALBS |
31.31 |
28.78 |
-2.53 |
| 2 |
Napoli, Julia R (15)
|
ADHAWK |
29.70 |
28.82 |
-0.88 |
| 3 |
Staudinger, Samuel P (13)
|
ADALBS |
34.35 |
30.45 |
-3.90 |
| 4 |
Riddle, Tiffany A (17)
|
ADUN |
35.49 |
30.92 |
-4.57 |
| 5 |
Shinaishin, Nabil A (15)
|
ADDELM |
33.96 |
32.31 |
-1.65 |
| 6 |
Ejanda, Francine Charisse A (13)
|
ADDELM |
33.97 |
32.47 |
-1.50 |
| 7 |
Wolfort, Madison J (11)
|
ADALBS |
33.82 |
33.95 |
0.13 |
| 8 |
O'Connell, Brodie R (13)
|
ADALBS |
48.95 |
39.60 |
-9.35 |
| 9 |
German, Levi J (10)
|
ADALBS |
49.94 |
44.87 |
-5.07 |
| 10 |
Hungerford, Cole B (9)
|
ADALBS |
55.14 |
49.07 |
-6.07 |
|
Donepudi, Gautam (14)
|
ADDELM |
1:09.21 |
DQ |
- |
|
Thela, Elizabeth M (13)
|
ADDELM |
|
DQ |
- |