| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Brown, Emma C (10)
|
IAMACR |
36.20 |
34.75 |
-1.45 |
| 2 |
Brown, Addison M (10)
|
IAMACR |
37.17 |
37.12 |
-0.05 |
| 3 |
Luck, Addison A (10)
|
IAMACR |
43.44 |
40.80 |
-2.64 |
| 4 |
Crist, Josie A (9)
|
IACRSP |
40.51 |
41.62 |
1.11 |
| 5 |
Goldberg, Elise (9)
|
IACRSP |
46.31 |
42.12 |
-4.19 |
| 6 |
Detterbeck, Chloe (10)
|
IAMACR |
42.02 |
42.26 |
0.24 |
| 7 |
Christensen, Rosilyn * (9)
|
IACRSP |
44.55 |
43.02 |
-1.53 |
| 8 |
Rens-Wasson, Lucy A (9)
|
IAMACR |
43.22 |
44.54 |
1.32 |
| 9 |
Smith, Shiloh (9)
|
IACRSP |
44.13 |
46.91 |
2.78 |
| 10 |
Newell, Josie (9)
|
IABUR |
50.11 |
52.43 |
2.32 |
| 11 |
Stadtmueller, Katie M (10)
|
IAMACR |
53.42 |
52.45 |
-0.97 |
| 12 |
Packard, Zoe (9)
|
IACRSP |
52.22 |
53.13 |
0.91 |
| 13 |
Otten, Olivia O (9)
|
IACRSP |
58.02 |
54.09 |
-3.93 |
|
Millsap, Morgan (9)
|
IAMAQ |
45.75 |
NS |
- |