| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Dunham, Julia E (10)
|
VAGATR |
40.11 |
34.12 |
-5.99 |
| 2 |
Osada, Elizabeth R (9)
|
VAACAC |
38.36 |
34.15 |
-4.21 |
| 3 |
Keller, Madison G (10)
|
VAGATR |
41.72 |
34.39 |
-7.33 |
| 4 |
Scherer, Anne T (10)
|
VAACAC |
34.36 |
34.53 |
0.17 |
| 5 |
Scherer, Hunter L (10)
|
VAACAC |
37.47 |
35.50 |
-1.97 |
| 6 |
Thompson, Isabelle R (10)
|
VAGATR |
38.69 |
36.07 |
-2.62 |
| 7 |
Reedy, Carly S (10)
|
VAGATR |
39.60 |
37.44 |
-2.16 |
| 8 |
Bing, Rachael C (10)
|
VAGATR |
40.55 |
37.52 |
-3.03 |
| 9 |
Hooke, Alison P (9)
|
VAGATR |
41.99 |
37.57 |
-4.42 |
| 10 |
McCardle, Reese (9)
|
VAACAC |
42.68 |
38.83 |
-3.85 |
| 11 |
Abdulaeva, Nicole (10)
|
VAACST |
|
41.62 |
- |
| 12 |
Douglas, Adeline M (10)
|
VAGATR |
42.38 |
43.38 |
1.00 |
| 13 |
Moseley, Kaitlyn S (9)
|
VAGATR |
47.80 |
45.31 |
-2.49 |
| 14 |
Loar, Sophia B (10)
|
VAACST |
|
52.68 |
- |
|
Trella-Luedtke, Isabel G (10)
|
VAACST |
|
NS |
- |
|
Anderson, Margaret J (9)
|
VAACST |
|
NS |
- |
|
Barclay, Sydney M (10)
|
VAACAC |
|
NS |
- |