| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Shafer, Naomi R (10)
|
VAGATR |
48.05 |
47.64 |
-0.41 |
| 2 |
Schwarzmann, Gianna G (9)
|
VASTRM |
53.00 |
47.82 |
-5.18 |
| 3 |
McConnell, Ansley G (10)
|
VAGATR |
55.76 |
49.72 |
-6.04 |
| 4 |
Miller, Keira A (10)
|
VAGATR |
|
50.38 |
- |
| 5 |
Kann, Jozelyn F (10)
|
VAGATR |
51.36 |
50.66 |
-0.70 |
| 6 |
Yang, Evelyn B (9)
|
VAGATR |
55.43 |
51.77 |
-3.66 |
| 7 |
Fuentes, Camille M (10)
|
VASTRM |
|
53.50 |
- |
| 8 |
Shrum, Calais A (9)
|
VAGATR |
59.51 |
55.27 |
-4.24 |
| 9 |
Kaltenborn, Victoria I (9)
|
VAGATR |
|
1:01.33 |
- |
| 10 |
Knick, Kayleigh B (10)
|
VASTRM |
|
1:06.43 |
- |
|
Brodfuehrer, Sienna (9)
|
VAVOLT |
|
DQ |
- |