| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Dinh, Benson (15)
|
NIGRSC |
4:34.43 |
4:47.12 |
12.69 |
| 2 |
Morreale, Alexander H (15)
|
NIHAST |
4:56.42 |
5:04.41 |
7.99 |
| 3 |
Kidder, Elizabeth A (15)
|
NIHAST |
4:57.50 |
5:10.71 |
13.21 |
| 4 |
Federico, Sydney A (16)
|
NIGRSC |
5:19.27 |
5:20.62 |
1.35 |
| 5 |
Jackel, Summer N (16)
|
NIGRSC |
5:18.61 |
5:21.89 |
3.28 |
| 6 |
Enser, Justin M (12)
|
NIHAST |
5:34.88 |
5:29.96 |
-4.92 |
| 7 |
Rich, Molly E (15)
|
NIHAST |
|
5:55.20 |
- |
| 8 |
Runyon, Jacob R (12)
|
NIHAST |
5:59.18 |
6:01.71 |
2.53 |
| 9 |
Robinson, Sara C (11)
|
NISDSC |
6:13.84 |
6:24.04 |
10.20 |
| 10 |
Heckle, Madelyn E (14)
|
NIGRSC |
|
6:33.61 |
- |
|
Antonini, Gianluca B (13)
|
NIHAST |
|
DQ |
- |