| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Blake, Emily C (12)
|
NISTAR |
27.50 |
27.58 |
0.08 |
| 2 |
Bauer, Lauren A (12)
|
NICLSC |
28.70 |
28.14 |
-0.56 |
| 3 |
Lee, Madeline E (12)
|
NIBAAC |
28.10 |
28.83 |
0.73 |
| 4 |
Martino, Adrianna L (12)
|
NIWWSC |
29.18 |
29.30 |
0.12 |
| 5 |
Fritsch, Natalie A (12)
|
NIBAAC |
29.44 |
29.67 |
0.23 |
| 6 |
Hall, Sofia F (12)
|
NIBAAC |
30.01 |
29.81 |
-0.20 |
| 7 |
Hall, Makaila (12)
|
NIVELO |
|
30.34 |
- |
| 8 |
Rude, Megan R (12)
|
NISHAQ |
33.45 |
30.61 |
-2.84 |
| 9 |
Shedd, Teagan E (12)
|
NISTAR |
30.16 |
30.88 |
0.72 |
| 10 |
McMullen, Haley (12)
|
NIVELO |
32.29 |
31.39 |
-0.90 |
| 11 |
Rietz, Tarin (12)
|
NIVELO |
31.77 |
31.51 |
-0.26 |
| 12 |
Zolet, Marta E (12)
|
NIEAST |
33.49 |
32.77 |
-0.72 |
| 13 |
Kowell-Ure, Phoenix W (12)
|
NIEAST |
32.46 |
32.81 |
0.35 |
| 14 |
Moore, Paige E (12)
|
NIUN-D |
33.59 |
33.01 |
-0.58 |
| 15 |
Vartanova, Sofia (12)
|
NIUN-D |
35.91 |
34.63 |
-1.28 |
| 16 |
Cronk, Sophia E (12)
|
NICLSC |
35.01 |
35.43 |
0.42 |
| 17 |
Hotchkiss, Olivia S (12)
|
NISTAR |
37.00 |
36.74 |
-0.26 |
| 18 |
Naim, Rita (12)
|
NIUN-D |
42.10 |
39.35 |
-2.75 |
| 19 |
Asher, Sophia (12)
|
NIVELO |
39.65 |
39.89 |
0.24 |
|
Canestaro, Annabelle S (12)
|
NIVELO |
41.43 |
DQ |
- |
|
Matyas, Morgan Q (12)
|
NISTAR |
27.59 |
NS |
- |
|
Fix, Grayce R (12)
|
NIWWSC |
42.64 |
NS |
- |
|
Krough, Mia S (12)
|
NICLSC |
40.07 |
NS |
- |