| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Blake, Emily C (12)
|
NISTAR |
27.98 |
29.82 |
1.84 |
| 2 |
Fritsch, Natalie A (12)
|
NIBAAC |
31.88 |
32.48 |
0.60 |
| 3 |
Hall, Sofia F (12)
|
NIBAAC |
34.06 |
33.31 |
-0.75 |
| 4 |
Moore, Kathleen E (12)
|
NIUN-D |
34.88 |
35.28 |
0.40 |
| 5 |
Moore, Paige E (12)
|
NIUN-D |
39.53 |
37.52 |
-2.01 |
| 6 |
Martino, Adrianna L (12)
|
NIWWSC |
37.30 |
39.02 |
1.72 |
| 7 |
Kowell-Ure, Phoenix W (12)
|
NIEAST |
41.74 |
39.35 |
-2.39 |
| 8 |
McMullen, Haley (12)
|
NIVELO |
37.96 |
41.11 |
3.15 |
| 9 |
Zolet, Marta E (12)
|
NIEAST |
48.54 |
47.83 |
-0.71 |
| 10 |
Hotchkiss, Olivia S (12)
|
NISTAR |
50.39 |
50.10 |
-0.29 |
| 11 |
Asher, Sophia (12)
|
NIVELO |
52.18 |
50.93 |
-1.25 |
| 12 |
Naim, Rita (12)
|
NIUN-D |
53.14 |
52.06 |
-1.08 |
| 13 |
Canestaro, Annabelle S (12)
|
NIVELO |
|
56.24 |
- |
|
Krough, Mia S (12)
|
NICLSC |
|
NS |
- |