| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Er, Melissa (9)
|
NIUN |
50.00 |
39.65 |
-10.35 |
| 2 |
Strittmatter, Amelia C (9)
|
NISTAR |
49.59 |
42.96 |
-6.63 |
| 3 |
Philbrick, Mylie E (9)
|
NIVELO |
52.88 |
47.57 |
-5.31 |
| 4 |
Barillari, Emma (9)
|
NIVELO |
45.82 |
48.10 |
2.28 |
| 5 |
Wamsley, Sarah E (9)
|
NIVELO |
51.46 |
50.15 |
-1.31 |
| 6 |
Gachette, Marisa B (9)
|
NIVELO |
57.76 |
51.98 |
-5.78 |
| 7 |
King, Skylar L (9)
|
NIEAST |
51.73 |
52.78 |
1.05 |
| 8 |
Weisenburg, Brylee M (9)
|
NISTAR |
59.87 |
57.96 |
-1.91 |
| 9 |
Reukauf, Anna R (9)
|
NISTAR |
1:00.53 |
1:03.69 |
3.16 |
| 10 |
Tothero, Madeleine S (9)
|
NISTAR |
1:01.84 |
1:07.06 |
5.22 |
| 11 |
Kauffman, Faith M (9)
|
NISTAR |
1:32.84 |
1:14.37 |
-18.47 |
|
Barrera-Hagner, Ananya S (9)
|
NIEAST |
|
NS |
- |