| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Keller, Mira (10)
|
NIVELO |
35.01 |
35.51 |
0.50 |
| 2 |
Reilly, Fiona K (10)
|
NISTAR |
38.70 |
36.32 |
-2.38 |
| 3 |
Chung, Emily (10)
|
NIBAAC |
39.29 |
39.23 |
-0.06 |
| 4 |
Pike, Olivia R (10)
|
NISTAR |
43.69 |
39.55 |
-4.14 |
| 5 |
Kreinheder, Sophie A (10)
|
NISTAR |
44.07 |
42.57 |
-1.50 |
| 6 |
Wands, Amelia G (10)
|
NIVELO |
45.34 |
43.61 |
-1.73 |
| 7 |
Kessel, Grace E (10)
|
NISHAQ |
47.41 |
44.92 |
-2.49 |
| 8 |
Vogt, Grace A (10)
|
NISTAR |
50.91 |
44.95 |
-5.96 |
| 9 |
Wilson, Aria C (10)
|
NIUN |
55.65 |
45.76 |
-9.89 |
| 10 |
Peck, Violet E (10)
|
NIEAST |
50.52 |
46.38 |
-4.14 |
| 11 |
Moldenhauer, Paige A (10)
|
NIEAST |
47.42 |
47.48 |
0.06 |
| 12 |
Vartanova, Elizaveta (10)
|
NIUN |
45.00 |
48.51 |
3.51 |
| 13 |
Cotter, Keara L (10)
|
NISTAR |
56.96 |
51.08 |
-5.88 |
| 14 |
Brassard, Addison G (10)
|
NISTAR |
51.30 |
51.41 |
0.11 |
| 15 |
Goergen, Vanessa C (10)
|
NIEAST |
58.06 |
51.98 |
-6.08 |
| 16 |
Rivera, Grace M (10)
|
NISTAR |
|
52.33 |
- |
| 17 |
DelRegno, Isabella M (10)
|
NIVELO |
|
54.09 |
- |
| 18 |
Hagel, Fiona K (10)
|
NISTAR |
1:01.75 |
58.80 |
-2.95 |
|
Renzoni, Olivia R (10)
|
NISTAR |
|
DQ |
- |
|
Chen, Jiayi (10)
|
NIBAAC |
|
NS |
- |
|
Bandemer, Hannah T (10)
|
NIBAAC |
|
NS |
- |