| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Reilly, Fiona K (10)
|
NISTAR |
42.84 |
41.36 |
-1.48 |
| 2 |
Wands, Amelia G (10)
|
NIVELO |
49.50 |
42.77 |
-6.73 |
| 3 |
Keller, Mira (10)
|
NIVELO |
43.68 |
44.03 |
0.35 |
| 4 |
Moldenhauer, Paige A (10)
|
NIEAST |
47.68 |
46.41 |
-1.27 |
| 5 |
Chung, Emily (10)
|
NIBAAC |
53.52 |
47.05 |
-6.47 |
| 6 |
Vogt, Grace A (10)
|
NISTAR |
1:07.94 |
48.85 |
-19.09 |
| 7 |
Pike, Olivia R (10)
|
NISTAR |
51.51 |
48.90 |
-2.61 |
| 8 |
Gentile, Olivia (10)
|
NIVELO |
59.28 |
50.28 |
-9.00 |
| 9 |
Wilson, Aria C (10)
|
NIUN |
1:01.12 |
50.89 |
-10.23 |
| 10 |
Brassard, Addison G (10)
|
NISTAR |
54.93 |
52.85 |
-2.08 |
| 11 |
DelRegno, Isabella M (10)
|
NIVELO |
|
1:03.27 |
- |
| 12 |
Peck, Violet E (10)
|
NIEAST |
1:10.09 |
1:04.05 |
-6.04 |
| 12 |
Goergen, Vanessa C (10)
|
NIEAST |
1:15.32 |
1:04.05 |
-11.27 |
| 14 |
Kessel, Grace E (10)
|
NISHAQ |
1:03.32 |
1:04.47 |
1.15 |
| 15 |
Hagel, Fiona K (10)
|
NISTAR |
1:14.44 |
1:12.10 |
-2.34 |
|
Kreinheder, Sophie A (10)
|
NISTAR |
|
DQ |
- |
|
Vartanova, Elizaveta (10)
|
NIUN |
50.00 |
DQ |
- |
|
Bandemer, Hannah T (10)
|
NIBAAC |
1:01.32 |
NS |
- |
|
Chen, Jiayi (10)
|
NIBAAC |
|
NS |
- |