| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Killion, Natalie A (12)
|
NICLSC |
37.19 |
34.00 |
-3.19 |
| 2 |
Leighton, Evelyn S (12)
|
NISTAR |
35.51 |
34.71 |
-0.80 |
| 3 |
Tesluk, Elizabeth D (12)
|
NISTAR |
36.10 |
37.10 |
1.00 |
| 4 |
Amer, Aya F (12)
|
NIVELO |
42.59 |
37.60 |
-4.99 |
| 5 |
Domster, Madeline N (12)
|
NISTAR |
41.60 |
38.59 |
-3.01 |
| 6 |
Pirigyi, Sophia J (12)
|
NISTAR |
40.82 |
40.47 |
-0.35 |
| 7 |
Berdysiak, Elizabeth C (12)
|
NICLSC |
39.99 |
40.62 |
0.63 |
| 8 |
Vaccaro, Vanessa A (12)
|
NIBAAC |
39.95 |
41.45 |
1.50 |
| 9 |
Lin, Isabella E (12)
|
NIVELO |
44.83 |
41.47 |
-3.36 |
| 10 |
Colder, Amelia W (12)
|
NISTAR |
43.66 |
42.61 |
-1.05 |
| 11 |
Pike, Olivia R (12)
|
NISTAR |
45.15 |
42.64 |
-2.51 |
| 12 |
Brown, Haley C (12)
|
NIVELO |
|
42.82 |
- |
| 13 |
Tothero, Katherine M (12)
|
NISTAR |
45.15 |
44.25 |
-0.90 |
| 14 |
Fosberg, Ella G (12)
|
NISTAR |
45.44 |
44.55 |
-0.89 |
| 15 |
Hull, Emily G (12)
|
NISTAR |
44.34 |
44.76 |
0.42 |
| 16 |
Morelli, Emily G (12)
|
NICLSC |
47.31 |
45.84 |
-1.47 |
| 17 |
Nadarajan, Gouripriya (12)
|
NIVELO |
|
48.37 |
- |
| 18 |
Denham, Myanna M (12)
|
NISTAR |
|
49.24 |
- |
| 19 |
Delille, Beatrice F (12)
|
NIVELO |
|
49.67 |
- |
| 20 |
Tripi, Haylee E (12)
|
NIVELO |
57.65 |
54.47 |
-3.18 |
|
Eisenmann, Abigail E (12)
|
NISTAR |
|
DQ |
- |