| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gahagan, Maggie G (9)
|
VAUN-D |
43.87 |
43.09 |
-0.78 |
| 2 |
Patel, Kayla G (10)
|
VATAC |
55.25 |
48.64 |
-6.61 |
| 3 |
Besachio, Alessandra L (10)
|
VATAC |
56.95 |
49.38 |
-7.57 |
| 4 |
Pflanz, Lilija M (9)
|
VADIG |
51.41 |
51.68 |
0.27 |
| 5 |
Lensegrav, Alejandra L (10)
|
VATAC |
58.58 |
51.94 |
-6.64 |
| 6 |
Sullivan, Faith E (10)
|
VADIG |
|
52.93 |
- |
| 7 |
Dixon, Elizabeth A (9)
|
VATAC |
|
53.12 |
- |
| 8 |
Morgan, Katrina E (10)
|
VATAC |
56.71 |
53.30 |
-3.41 |
| 9 |
Hinton, Emily L (9)
|
VADIG |
1:07.16 |
57.66 |
-9.50 |
| 10 |
Jenkins, Morgan W (9)
|
VADIG |
|
58.71 |
- |
| 11 |
Gage, Hanna A (9)
|
VATAC |
1:11.55 |
59.59 |
-11.96 |
| 12 |
Beasley, Marion A (9)
|
VATAC |
|
1:03.90 |
- |