| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Knepper, Sophie N (12)
|
VAUN-D |
26.96 |
27.77 |
0.81 |
| 2 |
Lensegrav, Ailana L (12)
|
VATAC |
32.72 |
30.97 |
-1.75 |
| 3 |
Alinsonorin, Bianca C (11)
|
VATAC |
31.45 |
31.30 |
-0.15 |
| 3 |
Scheneman, Jacqueline B (11)
|
VATAC |
32.06 |
31.30 |
-0.76 |
| 5 |
Callan, Lindsey F (12)
|
VAUN-D |
29.40 |
31.52 |
2.12 |
| 6 |
Winston, Isabella C (12)
|
VATAC |
33.73 |
32.04 |
-1.69 |
| 7 |
Price, Marlin G (11)
|
VAUN-D |
32.33 |
32.51 |
0.18 |
| 8 |
Digby, Brianna L (11)
|
VAUN-D |
33.40 |
33.87 |
0.47 |
| 9 |
Ashby, Leah P (11)
|
VADIG |
|
34.45 |
- |
| 10 |
Nguyen, Lauren K (11)
|
VATAC |
37.36 |
35.82 |
-1.54 |
| 11 |
Straley, Abigail F (11)
|
VAUN-D |
44.09 |
39.26 |
-4.83 |
|
Harrison, Tristan T (12)
|
VADIG |
|
NS |
- |
|
Kelly, CatherineRose W (12)
|
VATAC |
36.72 |
NS |
- |