| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Barros, Debora (13)
|
NIUN |
|
29.34 |
- |
| 2 |
Gioia, Abigail C (13)
|
NITTSC |
31.55 |
32.41 |
0.86 |
| 3 |
Roberts, Alaina G (13)
|
NITTSC |
33.60 |
32.73 |
-0.87 |
| 4 |
Kubiak, Kelly A (13)
|
NITWST |
34.67 |
32.74 |
-1.93 |
| 5 |
Kamens, Madelyne H (14)
|
NITTSC |
33.86 |
33.12 |
-0.74 |
| 6 |
Xu, Emily (14)
|
NIVELO |
|
34.18 |
- |
| 7 |
Drews, Maren G (13)
|
NITTSC |
36.02 |
34.71 |
-1.31 |
| 8 |
Mulawka, Paige M (13)
|
NIHAMB |
34.70 |
35.30 |
0.60 |
| 9 |
Rath, Cecilia R (14)
|
NICLSC |
|
35.79 |
- |
| 10 |
Heinrich, Chloe M (14)
|
NITWST |
40.74 |
36.76 |
-3.98 |
| 11 |
Chaplin, Allison V (13)
|
NITTSC |
36.63 |
36.99 |
0.36 |
| 12 |
Clouser, Kristiana I (13)
|
NIBCSR |
42.50 |
38.39 |
-4.11 |
| 13 |
Pauly, Hannah R (13)
|
NIFALC |
41.49 |
39.51 |
-1.98 |
| 14 |
Carden, Cassandra J (13)
|
NITWST |
40.41 |
39.84 |
-0.57 |
| 15 |
Polek, Sarah G (13)
|
NITTSC |
42.19 |
41.13 |
-1.06 |
| 16 |
Judy, Paris M (13)
|
NITWST |
1:09.19 |
43.26 |
-25.93 |
| 17 |
Canestaro, Annabelle S (13)
|
NIVELO |
56.24 |
44.21 |
-12.03 |
| 18 |
Stedge, Jocelyn E (13)
|
NIFALC |
52.68 |
45.23 |
-7.45 |
| 19 |
Morrison, Emily L (14)
|
NITWST |
|
45.76 |
- |
| 20 |
Blank, Sabrina E (13)
|
NITWST |
53.35 |
48.65 |
-4.70 |
| 21 |
Hammond, Taylor M (13)
|
NIEMAC |
54.24 |
52.07 |
-2.17 |
|
Higley, Kayla P (14)
|
NIRAYS |
|
DQ |
- |
|
Brodnicki, Brielle T (14)
|
NIVELO |
31.65 |
NS |
- |