| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kashino, Thomas S (14)
|
NISTAR |
31.97 |
28.82 |
-3.15 |
| 1 |
Strittmatter, Jude A (13)
|
NISTAR |
29.75 |
28.82 |
-0.93 |
| 3 |
Adams, Marshall B (13)
|
NITWST |
31.53 |
30.87 |
-0.66 |
| 4 |
King, Declan A (13)
|
NITTSC |
32.80 |
33.16 |
0.36 |
| 5 |
Kjellstrom, Anders J (13)
|
NITWST |
34.27 |
33.21 |
-1.06 |
| 6 |
Waldron, Keller D (13)
|
NITTSC |
38.51 |
34.10 |
-4.41 |
| 7 |
Domster, Aidan M (13)
|
NISTAR |
36.20 |
36.29 |
0.09 |
| 8 |
Noecker, Eli M (13)
|
NITWST |
35.80 |
36.58 |
0.78 |
| 9 |
Gorney, Caleb A (14)
|
NITTSC |
39.07 |
37.89 |
-1.18 |
| 10 |
Kopp, Daenon M (13)
|
NITTSC |
40.94 |
39.02 |
-1.92 |
| 11 |
Schoenborn, Cole D (13)
|
NICLSC |
|
42.29 |
- |
| 12 |
Dana, Gus R (14)
|
NISTAR |
|
43.03 |
- |
| 13 |
Lesmeister, Nicholas M (13)
|
NITTSC |
44.75 |
44.09 |
-0.66 |
|
O'Beirne, Brendan A (13)
|
NISTAR |
39.94 |
NS |
- |
|
Foley, Samuel J (14)
|
NITWST |
59.10 |
NS |
- |