| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Shunk, Morgan S (8)
|
NICLSC |
58.62 |
54.27 |
-4.35 |
| 2 |
Rasmusson, Henrietta E (8)
|
NIUN-F |
57.40 |
56.83 |
-0.57 |
| 3 |
Clouser, Kerrington D (8)
|
NIBCSR |
57.07 |
58.56 |
1.49 |
| 4 |
Loehfelm, Haley E (8)
|
NITWST |
58.12 |
59.37 |
1.25 |
| 5 |
Malkiewicz, Heather K (8)
|
NITTSC |
|
59.53 |
- |
| 6 |
Idzik, Hannah K (8)
|
NIFALC |
1:00.24 |
1:01.52 |
1.28 |
| 7 |
Attuwaybi, Eman (8)
|
NIVELO |
|
1:03.69 |
- |
| 8 |
Attuwaybi, Hibah (7)
|
NIVELO |
|
1:04.36 |
- |
| 9 |
Amer, Sarah (8)
|
NIVELO |
|
1:04.96 |
- |
| 10 |
Baker, Catherine M (7)
|
NITWST |
|
1:08.34 |
- |
| 11 |
Rogalski, Siena A (8)
|
NIBAAC |
|
1:11.70 |
- |
| 12 |
Loehfelm, Madelyn G (6)
|
NITWST |
|
1:23.82 |
- |
|
Wager, Allyson M (6)
|
NITTSC |
|
DQ |
- |
|
Reigelman, Allison B (7)
|
NITTSC |
|
NS |
- |