| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Alexander, Reece E (17)
|
NCTYDE |
53.27 |
55.37 |
2.10 |
| 2 |
Cottingham, Brianna A (16)
|
NCTYDE |
54.39 |
56.28 |
1.89 |
| 3 |
Klosterman, Carstyn J (17)
|
NCTYDE |
51.61 |
57.05 |
5.44 |
| 4 |
Wiles, Morgan E (15)
|
NCTYDE |
56.32 |
57.46 |
1.14 |
| 5 |
Mohr, Grace K (16)
|
NCTYDE |
53.97 |
57.63 |
3.66 |
| 6 |
Jennings, Abby C (17)
|
NCTYDE |
57.08 |
58.85 |
1.77 |
| 7 |
Gladden, Carlin R (17)
|
NCTYDE |
1:01.47 |
1:00.62 |
-0.85 |
| 8 |
Smith, Kelly M (16)
|
NCTYDE |
1:05.52 |
1:04.05 |
-1.47 |
| 9 |
Smith, Ellison F (15)
|
NCTYDE |
1:04.83 |
1:04.70 |
-0.13 |
| 10 |
Wells, Elise M (15)
|
NCTYDE |
1:24.37 |
1:24.29 |
-0.08 |