| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cribbs, Mila E (12)
|
AMPRA |
2:50.89 |
2:52.70 |
1.81 |
| 2 |
Fritsch, Natalie A (12)
|
NIUN-T |
2:50.19 |
2:53.73 |
3.54 |
| 3 |
Connelly, Riley A (12)
|
AMPRA |
2:53.58 |
2:54.48 |
0.90 |
| 4 |
Cordle, Gracie A (12)
|
NIFALC |
|
3:15.77 |
- |
| 5 |
Clouser, Kristiana I (12)
|
NIBCSR |
3:36.37 |
3:15.85 |
-20.52 |
| 6 |
Berdysiak, Elizabeth C (12)
|
NICLSC |
|
3:20.06 |
- |
| 7 |
Anderson, Ariana A (11)
|
AMPRA |
3:14.76 |
3:27.82 |
13.06 |
| 8 |
Griffin, Haley A (11)
|
NITTSC |
3:24.78 |
3:28.73 |
3.95 |
| 9 |
Del Regno, Jaina R (12)
|
NIRAYS |
|
3:34.02 |
- |
| 10 |
Mitchell, Gwenyth E (11)
|
NIEMAC |
3:16.90 |
3:34.14 |
17.24 |
| 11 |
Mruzik, Lillian G (11)
|
NIEAST |
|
3:40.76 |
- |
| 12 |
Moldenhauer, Paige A (11)
|
NIEAST |
|
3:46.43 |
- |
| 13 |
Zaccarine, Brooke D (12)
|
NIRAYS |
|
3:47.12 |
- |
| 14 |
Cameron, Nzingha A (12)
|
NIBCSR |
4:24.41 |
4:04.11 |
-20.30 |