| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Prusaczyk, Victoria (16)
|
NITWST |
3:07.47 |
3:15.41 |
7.94 |
| 2 |
Dueringer, Halle M (17)
|
NITWST |
3:12.59 |
3:15.61 |
3.02 |
| 3 |
Sapio, Isabel J (16)
|
NITWST |
2:49.76 |
3:20.98 |
31.22 |
| 4 |
Conley, Sarah L (15)
|
NITWST |
3:22.62 |
3:20.99 |
-1.63 |
| 5 |
Kenney, Sierra J (18)
|
NITWST |
3:06.67 |
3:21.20 |
14.53 |
| 6 |
Jaszcz, Madisyn P (19)
|
NITWST |
3:10.53 |
3:25.30 |
14.77 |
| 7 |
May, Julia A (16)
|
NIEAST |
3:23.49 |
3:25.77 |
2.28 |
| 8 |
Cordle, Gracie A (12)
|
NIFALC |
3:19.65 |
3:28.76 |
9.11 |
| 9 |
Carlson, Sarah E (12)
|
NIFALC |
3:41.41 |
3:34.50 |
-6.91 |
| 10 |
Faust, Ariel (15)
|
NIVELO |
3:21.95 |
3:38.67 |
16.72 |
| 11 |
Kisker, Elizabeth M (16)
|
NITWST |
3:41.31 |
3:42.70 |
1.39 |
|
Clouser, Kerrington D (8)
|
NIBCSR |
|
NS |
- |