| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Smith, Megan A (14)
|
AMPRA |
30.30 |
30.10 |
-0.20 |
| 2 |
Mikulec, Isabella J (13)
|
NITWST |
30.75 |
30.58 |
-0.17 |
| 3 |
Forcucci, Angela (13)
|
NITWST |
33.90 |
32.10 |
-1.80 |
| 4 |
Kohler, Anna C (13)
|
AMPRA |
32.29 |
32.77 |
0.48 |
| 5 |
Bissett, Ainsley L (14)
|
AMPRA |
31.44 |
32.87 |
1.43 |
| 6 |
Bautz, Michelina P (14)
|
NITWST |
31.35 |
32.91 |
1.56 |
| 7 |
Chugh, Katie F (13)
|
NIEAST |
32.53 |
32.92 |
0.39 |
| 8 |
Wiegand, Bailey M (14)
|
NITTSC |
31.05 |
33.11 |
2.06 |
| 9 |
Manczka, Madeline C (13)
|
AMPRA |
33.12 |
33.29 |
0.17 |
| 10 |
Gioia, Abigail C (13)
|
NITTSC |
31.47 |
33.44 |
1.97 |
| 11 |
Yang, Ellia (14)
|
NIVELO |
31.63 |
33.56 |
1.93 |
| 12 |
Spacciapolli, Victoria A (13)
|
NIUN |
33.01 |
34.21 |
1.20 |
| 13 |
Hesch, Natalie G (13)
|
NITTSC |
32.81 |
34.85 |
2.04 |
| 14 |
Teminski, Lucy (14)
|
BROC |
35.14 |
34.92 |
-0.22 |
| 15 |
Corriveau, Gabryelle (14)
|
BROC |
34.13 |
35.48 |
1.35 |
| 16 |
Roberts, Alaina G (13)
|
NITTSC |
33.66 |
35.63 |
1.97 |
| 17 |
Rath, Cecilia R (13)
|
NICLSC |
|
36.11 |
- |
| 18 |
Rietz, Tarin (13)
|
NIVELO |
34.09 |
36.34 |
2.25 |
| 19 |
Alexander, Sydney (13)
|
BROC |
33.73 |
36.43 |
2.70 |
| 20 |
Crane, Charley (13)
|
BROC |
32.87 |
36.66 |
3.79 |
| 21 |
Chaplin, Allison V (13)
|
NITTSC |
|
36.85 |
- |
| 22 |
Clouser, KaRynn F (14)
|
NIBCSR |
34.81 |
37.46 |
2.65 |
| 23 |
Standish, Madelyn O (13)
|
NICLSC |
|
38.65 |
- |
| 24 |
Malkiewicz, Claire E (13)
|
NITTSC |
38.09 |
39.90 |
1.81 |
| 25 |
Carden, Cassandra J (13)
|
NITWST |
36.97 |
39.98 |
3.01 |
| 26 |
Whitman, Mercy (14)
|
BROC |
34.96 |
40.30 |
5.34 |
| 27 |
Higley, Kayla P (13)
|
NIRAYS |
42.95 |
40.33 |
-2.62 |
| 28 |
Alakas, Tatiana (14)
|
BROC |
37.50 |
41.81 |
4.31 |
| 29 |
Enns, Madeleine (13)
|
BROC |
46.88 |
47.31 |
0.43 |
|
McNamara-Webster, Kaitlyn R (14)
|
NICLSC |
|
NS |
- |