| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wamsley, William H (14)
|
NIVELO |
27.11 |
27.56 |
0.45 |
| 2 |
DeSimone, Aidan A (13)
|
NCMOR |
27.79 |
28.47 |
0.68 |
| 3 |
Wilson, Christian (13)
|
NITWST |
28.87 |
29.56 |
0.69 |
| 4 |
Sapio, Luke T (14)
|
NITWST |
29.32 |
29.63 |
0.31 |
| 5 |
Coyne, Kiernan P (14)
|
NITWST |
31.06 |
31.11 |
0.05 |
| 6 |
Kisker, Benjamin J (13)
|
NITWST |
|
31.45 |
- |
| 7 |
Marshall, Parker T (13)
|
NIVELO |
29.94 |
31.78 |
1.84 |
| 8 |
Fritsch, Victor E (13)
|
NITTSC |
31.18 |
32.16 |
0.98 |
| 9 |
Attea, Phillip (13)
|
NIVELO |
31.62 |
32.91 |
1.29 |
| 10 |
Wangelin, Charlie L (14)
|
NITWST |
31.46 |
33.02 |
1.56 |
| 11 |
Landon, Tyler C (14)
|
NICLSC |
|
33.04 |
- |
| 12 |
Bishop, Blake (14)
|
BROC |
32.06 |
33.24 |
1.18 |
| 13 |
Fidler, Patrick (14)
|
BROC |
31.69 |
33.31 |
1.62 |
| 14 |
Cich, Charlie C (14)
|
NIEAST |
37.36 |
34.37 |
-2.99 |
| 15 |
Woodruff, Nicholas (13)
|
BROC |
35.43 |
34.39 |
-1.04 |
| 16 |
Signore, Christopher A (13)
|
NITTSC |
36.51 |
37.21 |
0.70 |
| 17 |
Schmitt, Jake (13)
|
NIVELO |
35.16 |
37.65 |
2.49 |
| 18 |
Gorney, Caleb A (13)
|
NITTSC |
39.53 |
37.82 |
-1.71 |
| 19 |
Ferron, Gabriel (13)
|
BROC |
38.23 |
37.88 |
-0.35 |
| 20 |
Steffens, Michael H (13)
|
NIRAYS |
1:05.99 |
40.90 |
-25.09 |
| 21 |
Campise, Kaden (13)
|
NITWST |
|
41.87 |
- |
| 22 |
Karaszewski, Zachary E (13)
|
NICLSC |
|
41.91 |
- |
|
Kersten, Tyler J (13)
|
NITWST |
|
NS |
- |