| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cribbs, Mila E (12)
|
AMPRA |
35.87 |
36.37 |
0.50 |
| 2 |
Connelly, Riley A (12)
|
AMPRA |
37.10 |
37.76 |
0.66 |
| 3 |
Yarnell, Natalie (12)
|
BROC |
39.38 |
40.46 |
1.08 |
| 4 |
Hulme, Samantha J (12)
|
NIEAST |
48.96 |
41.76 |
-7.20 |
| 5 |
Anderson, Ariana A (11)
|
AMPRA |
40.76 |
42.51 |
1.75 |
| 6 |
Mitchell, Gwenyth E (11)
|
NIEMAC |
42.46 |
43.29 |
0.83 |
| 7 |
Kowell-Ure, Phoenix W (12)
|
NIEAST |
44.02 |
43.36 |
-0.66 |
| 8 |
Mruzik, Lillian G (11)
|
NIEAST |
|
44.18 |
- |
| 9 |
Sainsbury, Meghan A (12)
|
NITWST |
50.52 |
45.48 |
-5.04 |
| 10 |
Sarginson, Piper (12)
|
BROC |
44.68 |
46.61 |
1.93 |
| 11 |
Del Regno, Jaina R (12)
|
NIRAYS |
52.56 |
46.94 |
-5.62 |
| 12 |
Berdysiak, Elizabeth C (12)
|
NICLSC |
43.68 |
47.33 |
3.65 |
| 13 |
Cartenuto, Katherine J (12)
|
NICLSC |
50.10 |
48.76 |
-1.34 |
| 14 |
Cimini, Ava M (11)
|
NIEAST |
58.04 |
50.11 |
-7.93 |
| 15 |
Bibler, Rachel L (11)
|
NICLSC |
|
51.34 |
- |
| 16 |
Cardina-Roden, Aubrey R (12)
|
NIEAST |
|
51.92 |
- |
| 17 |
Moldenhauer, Paige A (11)
|
NIEAST |
55.02 |
52.16 |
-2.86 |
| 18 |
Zaccarine, Brooke D (12)
|
NIRAYS |
|
52.35 |
- |
| 19 |
Battistoni, Grace L (11)
|
NIVELO |
|
53.87 |
- |
| 20 |
Hauser-Howells, Lillian K (12)
|
NITTSC |
57.24 |
55.69 |
-1.55 |
| 21 |
Ntiranyibagira, Parfaite M (11)
|
NIRAYS |
|
1:04.76 |
- |