| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Krumpek, Ella E (10)
|
NITWST |
52.47 |
50.59 |
-1.88 |
| 2 |
Ramos, Hannah M (10)
|
NIRAYS |
52.33 |
51.56 |
-0.77 |
| 3 |
Rados, Lucy M (10)
|
NITWST |
51.51 |
52.00 |
0.49 |
| 4 |
Zinno, Gianna M (10)
|
NICLSC |
|
52.16 |
- |
| 5 |
Scibetta, Madeline (10)
|
NITTSC |
53.10 |
52.55 |
-0.55 |
| 6 |
Wilkie, Kaitlin G (10)
|
NIRAYS |
57.65 |
56.84 |
-0.81 |
| 7 |
Winker, Delilah M (9)
|
NITTSC |
|
57.96 |
- |
| 8 |
Cribbs, Willow R (9)
|
AMPRA |
1:04.69 |
58.40 |
-6.29 |
| 9 |
Roberts, Meredith J (8)
|
NITTSC |
1:02.14 |
58.45 |
-3.69 |
| 10 |
Loehfelm, Haley E (9)
|
NITWST |
56.90 |
1:01.18 |
4.28 |
| 11 |
Malkiewicz, Heather K (9)
|
NITTSC |
1:08.70 |
1:01.40 |
-7.30 |
| 12 |
Rasmusson, Henrietta E (8)
|
NIFALC |
1:07.59 |
1:05.18 |
-2.41 |
| 13 |
Lyons, Lucy E (10)
|
NITTSC |
57.51 |
1:08.38 |
10.87 |
| 14 |
Fritsch, Lauren P (8)
|
NITTSC |
1:13.08 |
1:12.12 |
-0.96 |
| 15 |
Cone, Sadie L (9)
|
NITTSC |
1:17.79 |
1:16.78 |
-1.01 |
| 16 |
Hesch, Addison L (10)
|
NITTSC |
1:29.45 |
1:22.80 |
-6.65 |
|
Piscitello, Aniella O (8)
|
NIRAYS |
|
DQ |
- |
|
Roeser, Lila J (10)
|
NITTSC |
1:07.09 |
DQ |
- |
|
Nuara, Gabrielle S (10)
|
NICLSC |
|
DQ |
- |
|
Griffin, Grace K (8)
|
NITTSC |
1:10.11 |
NS |
- |