| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Branch, Alison G (17)
|
NIIRON |
26.61 |
27.59 |
0.98 |
| 2 |
Patterson, Ellen R (15)
|
NIIRON |
28.40 |
27.95 |
-0.45 |
| 3 |
Keyes, Angelina T (13)
|
NIGRSC |
26.50 |
28.17 |
1.67 |
| 4 |
DiFante, Marlayna R (17)
|
NIGCOM |
27.48 |
28.47 |
0.99 |
| 5 |
Salonen-Roperti, Pearl M (15)
|
NIGCOM |
29.02 |
29.87 |
0.85 |
| 6 |
Sak, Eleanor G (13)
|
NIGRSC |
35.19 |
30.65 |
-4.54 |
| 7 |
Polvino, Emilee E (16)
|
NIGRSC |
31.60 |
30.80 |
-0.80 |
| 8 |
McNamara, Grace E (15)
|
NIGRSC |
|
30.84 |
- |
| 9 |
Zona, Gianna I (13)
|
NIGRSC |
30.16 |
30.88 |
0.72 |
| 10 |
Camman, Kylie L (15)
|
NIGRSC |
30.88 |
31.21 |
0.33 |
| 11 |
Salonen-Roperti, Marissa A (17)
|
NIGCOM |
30.07 |
32.31 |
2.24 |
| 12 |
McBeth, Grace (13)
|
NISDSC |
34.01 |
32.85 |
-1.16 |
| 13 |
Kleiner, Hannah S (13)
|
NIGCOM |
|
34.17 |
- |
| 14 |
Rouse, Ellie R (13)
|
NISDSC |
34.81 |
34.68 |
-0.13 |
| 15 |
O'Connor, Molly C (14)
|
NIGRSC |
|
37.75 |
- |
|
Lesniak, Isabel R (14)
|
NIGCOM |
31.96 |
NS |
- |
|
Hann, Hailey E (13)
|
NICSCI |
30.89 |
NS |
- |
|
Thompson, Daniella J (14)
|
NIGRSC |
34.41 |
NS |
- |