| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Squires, Timothy D (23)
|
STASC |
50.92 |
50.57 |
-0.35 |
| 2 |
Schumacher, Griffin T (22)
|
MICW |
50.53 |
51.44 |
0.91 |
| 3 |
Al-Yamani, Mokhtar (18)
|
MIMICH |
51.80 |
51.75 |
-0.05 |
| 4 |
White, Justin R (20)
|
ILUN-0 |
52.35 |
52.30 |
-0.05 |
| 5 |
Schroeder, Hans A (18)
|
FLSYS |
52.30 |
52.38 |
0.08 |
| 6 |
Wagner, Zachary C (21)
|
PNKING |
52.19 |
52.57 |
0.38 |
| 7 |
Vukic, Mihael (21)
|
CCCSUB |
52.21 |
52.64 |
0.43 |
| 8 |
Newland, Nolan N (21)
|
CACPSU |
52.96 |
52.77 |
-0.19 |
| 9 |
Glegg, Ross S (23)
|
WVUN-0 |
43.96 |
52.80 |
8.84 |
| 10 |
Kwiecien-Delaney, Bryce J (19)
|
MIMICH |
50.71 |
52.81 |
2.10 |
| 11 |
Williams, Kai V (18)
|
MIMICH |
52.66 |
52.91 |
0.25 |
| 12 |
Trump, Trent A (19)
|
AZGCU |
52.07 |
52.99 |
0.92 |
| 13 |
Lee, Hohyun (18)
|
CAFAST |
51.30 |
53.06 |
1.76 |
| 14 |
Koller, Sebastian J (19)
|
OHWSU |
53.00 |
53.07 |
0.07 |
| 15 |
Woodman, David M (24)
|
USUC |
51.11 |
53.18 |
2.07 |
| 16 |
Olsen-Stavrakas, Kent H (24)
|
COUN-0 |
52.12 |
53.28 |
1.16 |
| 17 |
Eaton, Colin T (22)
|
MIMICH |
51.57 |
53.39 |
1.82 |
| 18 |
Timonera, Bryce P (20)
|
CCCSUB |
52.99 |
53.41 |
0.42 |
| 19 |
Goethe, Jackson P (20)
|
MIMICH |
52.19 |
53.42 |
1.23 |
| 20 |
Moss, Armando N (23)
|
OHXAV |
52.15 |
53.45 |
1.30 |
| 21 |
Willis, Jamal E (20)
|
MAUN-0 |
52.79 |
53.84 |
1.05 |
| 22 |
Van Krimpen, Owen (18)
|
CCCSUB |
53.26 |
54.53 |
1.27 |
| 23 |
Ghiglieri, Aaron M (22)
|
MIMICH |
52.70 |
54.62 |
1.92 |
| 24 |
Zimmerman, Matthew L (22)
|
MIMICH |
53.09 |
55.07 |
1.98 |
|
Mangan, Jack F (20)
|
MIMICH |
50.45 |
NS |
- |