| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Deuel, Megan G (16)
|
NIVICT |
24.45 |
25.49 |
1.04 |
| 2 |
Van Vreede, Olivia L (14)
|
NIVICT |
25.68 |
26.34 |
0.66 |
| 3 |
Sacco, Kaili C (16)
|
NIJETS |
26.86 |
26.57 |
-0.29 |
| 4 |
Hawkins, Emma (15)
|
NIVICT |
26.62 |
26.60 |
-0.02 |
| 5 |
Senglaub, Emma (15)
|
NIVICT |
26.74 |
26.73 |
-0.01 |
| 6 |
Pathammavong, Suriyathai A (14)
|
NIVICT |
25.90 |
26.83 |
0.93 |
| 7 |
LeFrois-Heath, Emily R (14)
|
NIVICT |
27.66 |
27.08 |
-0.58 |
| 8 |
Konz, Ashley L (14)
|
NIJETS |
26.50 |
27.10 |
0.60 |
| 9 |
McRae, Kathryn E (13)
|
NIVICT |
28.90 |
27.83 |
-1.07 |
| 10 |
Senglaub, Katie (15)
|
NIVICT |
27.53 |
27.90 |
0.37 |
| 11 |
Christodaro, Melina A (17)
|
NIHAST |
26.93 |
28.00 |
1.07 |
| 12 |
Miller, Alayna E (14)
|
NIHAST |
28.11 |
28.51 |
0.40 |
| 13 |
Burke, Clare E (16)
|
NIJETS |
27.78 |
29.03 |
1.25 |
| 14 |
Brooks, Kyleigh N (15)
|
NIGRAT |
|
29.42 |
- |
| 15 |
Hunter, Kerry A (13)
|
NIJETS |
29.94 |
29.65 |
-0.29 |
| 16 |
Newsome, Amber G (13)
|
NIPMSC |
29.60 |
30.09 |
0.49 |
| 17 |
Kazmark, Raelyn J (14)
|
NIVICT |
29.39 |
30.19 |
0.80 |
| 18 |
Barry, Madeline G (13)
|
NIVICT |
31.05 |
30.30 |
-0.75 |
| 19 |
Geoca, Zoey L (16)
|
NISDSC |
29.43 |
30.34 |
0.91 |
| 20 |
Berdysiak, Elizabeth C (13)
|
NICLSC |
30.55 |
30.51 |
-0.04 |
| 21 |
Kazmark, Olivia N (13)
|
NIVICT |
31.29 |
30.89 |
-0.40 |
| 22 |
Bugbee, Kyla C (13)
|
NIPMSC |
30.70 |
31.30 |
0.60 |
| 23 |
Thorne, Lillian E (14)
|
NIJETS |
29.76 |
32.03 |
2.27 |
| 24 |
Hayes, Sydney N (13)
|
NIJETS |
33.41 |
33.65 |
0.24 |
| 25 |
Christodaro, Raena M (14)
|
NIHAST |
32.69 |
33.92 |
1.23 |
| 26 |
McBeth, Grace (13)
|
NISDSC |
|
34.01 |
- |
| 27 |
Kenny, Elisabeth A (13)
|
NIVICT |
37.77 |
34.76 |
-3.01 |
|
Jessie, Rachel E (14)
|
NIJETS |
33.75 |
NS |
- |
|
Werner, Riley K (14)
|
NIVICT |
32.32 |
NS |
- |