| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Geary, Maria E (9)
|
NIMERC |
42.66 |
42.40 |
-0.26 |
| 2 |
Manciocchi, Kayden M (10)
|
NIHAST |
41.59 |
43.27 |
1.68 |
| 3 |
Giles, Jenna L (10)
|
NIGRAT |
48.48 |
47.71 |
-0.77 |
| 4 |
Van Hout, Kennyn A (9)
|
NISDSC |
49.20 |
48.72 |
-0.48 |
| 5 |
Corcoran, Riley A (10)
|
NISDSC |
55.82 |
51.84 |
-3.98 |
| 6 |
Ramsperger, Lily A (8)
|
NISDSC |
58.04 |
53.71 |
-4.33 |
| 7 |
Crowley, Lila M (10)
|
NIGRSC |
57.22 |
53.91 |
-3.31 |
| 8 |
Schneider, Brooke J (10)
|
NIMERC |
1:04.65 |
57.31 |
-7.34 |
| 9 |
Dickerson, Alice S (10)
|
NIHAST |
|
59.09 |
- |
|
Ayers, Brianna S (9)
|
NIGRSC |
|
DQ |
- |
|
Bermudez, Airianna L (10)
|
NIGRSC |
|
DQ |
- |
|
Cosner, Brynn S (10)
|
NIGRSC |
|
DQ |
- |
|
Tabor, Chloe E (10)
|
NIGCOM |
55.44 |
NS |
- |
|
Gavigan, Rosaleigh E (9)
|
NIHAST |
|
NS |
- |