| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cywinski, Anna C (9)
|
NIVICT |
36.05 |
37.75 |
1.70 |
| 2 |
Tytler, Elizabeth (10)
|
NIIRON |
38.34 |
39.99 |
1.65 |
| 3 |
Knausz, Eleanor M (10)
|
NISDSC |
40.40 |
40.87 |
0.47 |
| 4 |
Cafarelli, Brianna N (10)
|
NIQUIK |
39.67 |
41.05 |
1.38 |
| 5 |
Onze, Emily S (10)
|
NIVICT |
42.14 |
45.42 |
3.28 |
| 6 |
Gilbert, Natalie J (9)
|
NIQUIK |
40.07 |
45.69 |
5.62 |
| 7 |
Resch, Rebecka J (10)
|
NIGRSC |
47.57 |
46.99 |
-0.58 |
| 8 |
Hauber, Riley A (9)
|
NISDSC |
48.79 |
50.51 |
1.72 |
| 9 |
Manciocchi, Kayden M (9)
|
NIHAST |
53.33 |
52.16 |
-1.17 |
| 10 |
Anthony, Jillian M (10)
|
NIPMSC |
45.96 |
52.45 |
6.49 |
| 11 |
Catalano, Emilee K (9)
|
NIGRAT |
|
54.81 |
- |
| 12 |
Barber, Cassandra L (10)
|
NIGRSC |
55.69 |
54.92 |
-0.77 |
| 13 |
Ashby, Emma M (9)
|
NISDSC |
55.21 |
55.65 |
0.44 |
| 14 |
Crowley, Lila M (9)
|
NIGRSC |
59.12 |
56.99 |
-2.13 |
| 15 |
Pogroszewski, Frances S (10)
|
NIGCOM |
|
1:00.11 |
- |
| 16 |
Marsh, Emma L (10)
|
NIGRSC |
|
1:10.00 |
- |
| 17 |
Thaney, Brigid G (9)
|
NIGRSC |
1:03.52 |
1:11.53 |
8.01 |
|
Farina, Samantha R (10)
|
NIGRSC |
|
DQ |
- |
|
Bub, Emily R (10)
|
NIGRSC |
|
DQ |
- |
|
Burke, Mary C (10)
|
NIHAST |
|
DQ |
- |
|
Beghini, Sofia A (10)
|
NIHAST |
1:16.76 |
DQ |
- |
|
Unawane, Siya S (10)
|
NIGCOM |
|
DQ |
- |