| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sanson, Charlotte E (12)
|
NIPMSC |
30.07 |
29.28 |
-0.79 |
| 2 |
Keyes, Angelina T (12)
|
NIGRSC |
29.40 |
29.29 |
-0.11 |
| 3 |
Guagliardo, Giovanna (11)
|
NIIRON |
30.09 |
29.66 |
-0.43 |
| 4 |
Coyle, Muriel I (12)
|
NIGCOM |
31.25 |
31.09 |
-0.16 |
| 5 |
Zona, Gianna I (12)
|
NIGRSC |
32.48 |
31.51 |
-0.97 |
| 6 |
Natemeier, Amelia E (11)
|
NIUN-V |
31.40 |
31.60 |
0.20 |
| 7 |
Hersh, Erin (11)
|
NIVICT |
32.75 |
32.84 |
0.09 |
| 8 |
Martin, Lauren G (12)
|
NISDSC |
|
33.02 |
- |
| 9 |
Bouwens, Emma L (11)
|
NIHAST |
34.06 |
33.15 |
-0.91 |
| 10 |
Herron, Ella S (12)
|
NIHAST |
34.31 |
33.79 |
-0.52 |
| 11 |
Newsome, Amber G (12)
|
NIPMSC |
35.01 |
34.40 |
-0.61 |
| 12 |
Werner, Riley K (12)
|
NIVICT |
33.22 |
34.57 |
1.35 |
| 13 |
Beutner, Ella R (11)
|
NIQUIK |
34.72 |
34.85 |
0.13 |
| 14 |
Bugbee, Kyla C (12)
|
NIPMSC |
33.74 |
35.64 |
1.90 |
| 15 |
Smolinski, Evelynn J (11)
|
NIHAST |
38.43 |
36.84 |
-1.59 |
| 16 |
Rouse, Ellie R (12)
|
NIGRAT |
|
38.65 |
- |
| 17 |
Saysamone, Leina H (11)
|
NIGCOM |
43.08 |
39.85 |
-3.23 |
| 18 |
Thurston, Lauren A (11)
|
NIGCOM |
42.14 |
41.59 |
-0.55 |
| 19 |
Mosier, Georgia R (11)
|
NIGRAT |
|
42.03 |
- |
| 20 |
Hackett, Grace C (11)
|
NIIRON |
45.21 |
42.70 |
-2.51 |
| 21 |
Stoos, Arianna E (11)
|
NIGRAT |
52.25 |
43.26 |
-8.99 |
| 22 |
Wilson, Taylor J (11)
|
NIHAST |
|
46.10 |
- |
|
Waters, Megan R (12)
|
NIGCOM |
47.69 |
NS |
- |