| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bouwens, Emma L (12)
|
NIHAST |
29.44 |
29.19 |
-0.25 |
| 2 |
Robinson, Sara C (12)
|
NISDSC |
30.17 |
29.72 |
-0.45 |
| 3 |
Knausz, Eleanor M (11)
|
NISDSC |
30.19 |
29.97 |
-0.22 |
| 4 |
Geary, Kaitlyn J (12)
|
NIMERC |
30.79 |
30.74 |
-0.05 |
| 5 |
Chen, Chloe Y (11)
|
NISDSC |
31.63 |
30.79 |
-0.84 |
| 6 |
Bennett, Olivia R (12)
|
NICAQY |
32.66 |
31.18 |
-1.48 |
| 7 |
Share, Elizabeth A (12)
|
NIPMSC |
30.87 |
32.02 |
1.15 |
| 7 |
Anthony, Jillian M (11)
|
NIPMSC |
34.80 |
32.02 |
-2.78 |
| 9 |
Smolinski, Evelynn J (12)
|
NIHAST |
33.23 |
32.20 |
-1.03 |
| 10 |
Priebe, Anna J (11)
|
NIPMSC |
38.09 |
32.64 |
-5.45 |
| 11 |
Schwandt, Natalie K (11)
|
NIGRAT |
32.29 |
32.73 |
0.44 |
| 12 |
Strausser, Cadence A (11)
|
NIGRAT |
35.56 |
34.99 |
-0.57 |
| 12 |
Munson, Sophie M (11)
|
NIGRSC |
36.30 |
34.99 |
-1.31 |
| 14 |
Greisch, Alisha D (11)
|
NIGRSC |
36.12 |
35.33 |
-0.79 |
| 15 |
Wagner, Keaghan J (11)
|
NISDSC |
35.86 |
35.35 |
-0.51 |
| 16 |
Resch, Rebecka J (11)
|
NIGRSC |
35.15 |
37.43 |
2.28 |
| 17 |
Nitti, Marissa R (11)
|
NIGRSC |
37.46 |
37.64 |
0.18 |
| 18 |
Mosier, Georgia R (12)
|
NIGRAT |
42.03 |
39.14 |
-2.89 |
| 19 |
Bub, Emily R (11)
|
NIGRSC |
45.04 |
43.84 |
-1.20 |
| 20 |
Diaz, Zamirah C (12)
|
NIHAST |
54.18 |
51.44 |
-2.74 |
| 21 |
Salton, Mia R (11)
|
NIHAST |
44.30 |
1:01.80 |
17.50 |