| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Knausz, Eleanor M (11)
|
NISDSC |
38.92 |
38.81 |
-0.11 |
| 1 |
Robinson, Sara C (12)
|
NISDSC |
37.23 |
38.81 |
1.58 |
| 3 |
Bouwens, Emma L (12)
|
NIHAST |
44.01 |
41.24 |
-2.77 |
| 4 |
Geary, Kaitlyn J (12)
|
NIMERC |
41.30 |
41.68 |
0.38 |
| 5 |
Smolinski, Evelynn J (12)
|
NIHAST |
45.00 |
42.83 |
-2.17 |
| 6 |
Anthony, Jillian M (11)
|
NIPMSC |
51.08 |
45.84 |
-5.24 |
| 7 |
Kuhlkin, Elizabeth C (12)
|
NIGCOM |
48.20 |
46.01 |
-2.19 |
| 8 |
Bennett, Olivia R (12)
|
NICAQY |
48.31 |
47.40 |
-0.91 |
| 9 |
Mosier, Georgia R (12)
|
NIGRAT |
58.73 |
52.66 |
-6.07 |
| 10 |
Salton, Mia R (11)
|
NIHAST |
54.85 |
54.87 |
0.02 |
| 11 |
Strausser, Cadence A (11)
|
NIGRAT |
55.85 |
55.64 |
-0.21 |