| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sanson, Charlotte E (12)
|
NIPMSC |
33.45 |
32.33 |
-1.12 |
| 2 |
Natemeier, Amelia E (11)
|
NIUN-V |
35.42 |
35.31 |
-0.11 |
| 3 |
Flanigen, Ruby J (11)
|
NIIRON |
35.91 |
36.31 |
0.40 |
| 4 |
Bouwens, Emma L (11)
|
NIHAST |
37.83 |
39.21 |
1.38 |
| 5 |
Martin, Lauren G (12)
|
NISDSC |
|
41.80 |
- |
| 6 |
Herron, Ella S (12)
|
NIHAST |
48.56 |
44.20 |
-4.36 |
| 7 |
Bugbee, Kyla C (12)
|
NIPMSC |
43.26 |
44.32 |
1.06 |
| 8 |
Newsome, Amber G (12)
|
NIPMSC |
45.04 |
44.75 |
-0.29 |
| 9 |
Beutner, Ella R (11)
|
NIQUIK |
42.24 |
47.29 |
5.05 |
| 10 |
Smolinski, Evelynn J (11)
|
NIHAST |
1:00.62 |
48.17 |
-12.45 |
| 11 |
Kuhlkin, Elizabeth C (11)
|
NIGCOM |
49.30 |
52.03 |
2.73 |
| 12 |
Thompson, Daniella J (12)
|
NIGRSC |
|
52.27 |
- |
|
Wilson, Taylor J (11)
|
NIHAST |
|
DQ |
- |
|
Saysamone, Leina H (11)
|
NIGCOM |
|
NS |
- |
|
Thurston, Lauren A (11)
|
NIGCOM |
58.83 |
NS |
- |