| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Amthor, Brandon T (17)
|
NISEAS |
55.34 |
55.22 |
-0.12 |
| 2 |
Murphy, Brayden (14)
|
NIVICT |
58.80 |
1:00.15 |
1.35 |
| 3 |
Rivera, Samuel T (13)
|
NIIRON |
1:03.11 |
1:00.72 |
-2.39 |
| 4 |
Turek, Alex J (15)
|
NIVICT |
1:02.82 |
1:01.63 |
-1.19 |
| 5 |
Smith, Tyler N (16)
|
NIUN-V |
1:02.49 |
1:01.74 |
-0.75 |
| 6 |
Dambra, Nicholas (14)
|
NISEAS |
1:04.29 |
1:02.16 |
-2.13 |
| 7 |
DiBitetto, Jack J (14)
|
NIHAST |
58.96 |
1:02.34 |
3.38 |
| 8 |
Frear, Garrett B (17)
|
NIIRON |
1:01.61 |
1:02.67 |
1.06 |
| 9 |
Porretta, Gerald A (13)
|
NIIRON |
1:02.03 |
1:02.89 |
0.86 |
| 10 |
Wihlen, Christopher M (14)
|
NIVICT |
1:01.04 |
1:05.53 |
4.49 |
| 11 |
Enser, Jared M (13)
|
NIHAST |
1:07.79 |
1:05.93 |
-1.86 |
| 12 |
Schultz, Colin J (15)
|
NIHAST |
1:07.22 |
1:07.60 |
0.38 |
| 13 |
Smith, Connor J (13)
|
NIUN-V |
1:14.55 |
1:13.19 |
-1.36 |
| 14 |
Cunningham, Dalton M (13)
|
NISEAS |
1:21.35 |
1:14.61 |
-6.74 |
| 15 |
Ciravola, Jayden T (13)
|
NIHAST |
1:37.52 |
1:35.17 |
-2.35 |
| 16 |
Monroe, Lucas J (11)
|
NIPMSC |
2:34.84 |
1:58.50 |
-36.34 |
|
Anthony, Jaydon T (11)
|
NIPMSC |
2:12.96 |
DQ |
- |