| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sanson, Charlotte E (12)
|
NIPMSC |
37.79 |
36.88 |
-0.91 |
| 2 |
Bouwens, Emma L (11)
|
NIHAST |
38.45 |
39.01 |
0.56 |
| 3 |
Newsome, Amber G (12)
|
NIPMSC |
40.88 |
39.81 |
-1.07 |
| 4 |
Hersh, Erin (11)
|
NIVICT |
40.48 |
41.95 |
1.47 |
| 5 |
Rouse, Ellie R (12)
|
NIGRAT |
|
42.94 |
- |
| 6 |
Smolinski, Evelynn J (11)
|
NIHAST |
42.78 |
43.61 |
0.83 |
| 7 |
Kuhlkin, Elizabeth C (11)
|
NIGCOM |
43.87 |
43.71 |
-0.16 |
| 8 |
Herron, Ella S (12)
|
NIHAST |
45.20 |
45.56 |
0.36 |
| 9 |
Beutner, Ella R (11)
|
NIQUIK |
47.19 |
46.60 |
-0.59 |
| 10 |
Thompson, Daniella J (12)
|
NIGRSC |
45.47 |
46.77 |
1.30 |
| 11 |
Stoos, Arianna E (11)
|
NIGRAT |
49.96 |
50.94 |
0.98 |
| 12 |
Mosier, Georgia R (11)
|
NIGRAT |
|
51.85 |
- |
| 13 |
Hackett, Grace C (11)
|
NIIRON |
52.98 |
54.03 |
1.05 |
| 14 |
Thurston, Lauren A (11)
|
NIGCOM |
53.66 |
55.12 |
1.46 |
| 15 |
Wilson, Taylor J (11)
|
NIHAST |
47.14 |
58.71 |
11.57 |
|
Bugbee, Kyla C (12)
|
NIPMSC |
47.61 |
DQ |
- |
|
Waters, Megan R (12)
|
NIGCOM |
56.47 |
NS |
- |
|
Saysamone, Leina H (11)
|
NIGCOM |
46.47 |
NS |
- |