| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Smith, Tyler N (16)
|
NIUN-V |
49.65 |
51.49 |
1.84 |
| 2 |
Regna, Alex J (17)
|
NISEAS |
52.83 |
52.27 |
-0.56 |
| 3 |
Richardson, Austin J (18)
|
NIOLSC |
54.17 |
52.81 |
-1.36 |
| 4 |
Thomas, Joshua D (15)
|
NIOLSC |
54.14 |
53.33 |
-0.81 |
| 5 |
Turek, Alex J (15)
|
NIVICT |
54.06 |
54.39 |
0.33 |
| 6 |
Murphy, Brayden (14)
|
NIVICT |
52.55 |
55.25 |
2.70 |
| 7 |
Dambra, Nicholas (14)
|
NISEAS |
1:01.86 |
56.18 |
-5.68 |
| 8 |
Porretta, Gerald A (13)
|
NIIRON |
58.77 |
56.37 |
-2.40 |
| 9 |
DiBitetto, Jack J (14)
|
NIHAST |
55.99 |
56.70 |
0.71 |
| 10 |
Frear, Garrett B (17)
|
NIIRON |
56.27 |
56.84 |
0.57 |
| 11 |
Zani, Samuel A (14)
|
NIIRON |
59.61 |
57.53 |
-2.08 |
| 12 |
Coyle, Samuel J (14)
|
NISEAS |
58.08 |
57.79 |
-0.29 |
| 13 |
Enser, Jared M (13)
|
NIHAST |
57.94 |
58.48 |
0.54 |
| 14 |
Wihlen, Christopher M (14)
|
NIVICT |
57.13 |
58.79 |
1.66 |
| 15 |
Page, Christopher M (16)
|
NIMERC |
1:00.77 |
58.93 |
-1.84 |
| 16 |
Riexinger, Colby J (14)
|
NISEAS |
1:00.66 |
59.17 |
-1.49 |
| 17 |
Godown, Elijah M (15)
|
NIPMSC |
56.87 |
59.87 |
3.00 |
| 18 |
Murray, Maxwell P (14)
|
NISDSC |
58.04 |
1:00.03 |
1.99 |
| 19 |
Michalowski, Connor J (14)
|
NIHAST |
1:00.49 |
1:00.15 |
-0.34 |
| 20 |
Donovan, Rylan (16)
|
NISEAS |
|
1:00.48 |
- |
| 21 |
Smith, Connor J (13)
|
NIUN-V |
58.69 |
1:00.57 |
1.88 |
| 22 |
Morgan, Phillip A (14)
|
NIMERC |
|
1:02.63 |
- |
| 23 |
Morreale, Alexander H (13)
|
NIHAST |
1:04.19 |
1:03.99 |
-0.20 |
| 24 |
Mueller, Christopher J (14)
|
NISDSC |
1:05.46 |
1:05.74 |
0.28 |
| 25 |
Derousie, Mitchell B (14)
|
NIHAST |
1:11.63 |
1:09.37 |
-2.26 |
| 26 |
Ciravola, Jayden T (13)
|
NIHAST |
1:11.78 |
1:11.18 |
-0.60 |
| 27 |
McCarthy, John E (10)
|
NISDSC |
|
1:33.49 |
- |
| 28 |
Pompeii, Joey J (13)
|
NIPMSC |
1:45.20 |
1:41.68 |
-3.52 |
| 29 |
Newsome, George W (9)
|
NIPMSC |
|
1:52.42 |
- |