| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Edwardson, Tori E (12)
|
OHLTPY |
39.26 |
37.90 |
-1.36 |
| 2 |
Yee, Sydney M (11)
|
OHLTPY |
41.59 |
41.74 |
0.15 |
| 3 |
Brinkman, Madelyn M (11)
|
OHDRY |
40.74 |
41.80 |
1.06 |
| 4 |
Reville, Maren A (11)
|
OHLTPY |
41.53 |
42.21 |
0.68 |
| 5 |
Beck, Katelynn S (12)
|
OHDRY |
|
43.45 |
- |
| 6 |
Davis, Hannah G (11)
|
OHLTPY |
47.24 |
44.88 |
-2.36 |
| 7 |
Gudla, Sankeertana * (12)
|
OHLTPY |
50.69 |
48.69 |
-2.00 |
| 8 |
Snider, Bridget E (11)
|
OHLTPY |
44.87 |
48.89 |
4.02 |
| 9 |
Heckman, Sophia M (12)
|
OHLTPY |
|
50.77 |
- |
| 10 |
Ginsburg, Lucy R (12)
|
OHDRY |
|
51.45 |
- |
| 11 |
Ganesh, Samiksha * (11)
|
OHLTPY |
54.75 |
52.92 |
-1.83 |
| 12 |
Sutton, Ainsley E (11)
|
OHLTPY |
55.61 |
52.95 |
-2.66 |
|
Claggett, Brookelyn O (11)
|
OHLTPY |
45.92 |
DQ |
- |