| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Jones, Layne C (16)
|
VACCA |
53.66 |
55.45 |
1.79 |
| 2 |
Douglas, Gabriella M (17)
|
VANOVA |
54.90 |
56.53 |
1.63 |
| 3 |
McLaughlin, Delaney L (16)
|
VACCA |
55.28 |
57.11 |
1.83 |
| 4 |
Walker, Emma J (17)
|
VAWAC |
53.41 |
57.85 |
4.44 |
| 5 |
O'Donnell, Erin R (16)
|
PVRMSC |
57.88 |
57.90 |
0.02 |
| 6 |
Parker, Rachel F (17)
|
VAWAC |
55.24 |
58.52 |
3.28 |
| 7 |
Hanks, Lindsay N (17)
|
VAWAC |
57.07 |
1:01.14 |
4.07 |
| 8 |
Simkins, Elizabeth J (16)
|
VACCA |
57.14 |
1:03.13 |
5.99 |
| 9 |
McCourt, Lauren E (15)
|
VAWAC |
1:01.11 |
1:04.43 |
3.32 |
| 10 |
Douglewicz, Sarah E (15)
|
VANOVA |
1:01.79 |
1:05.18 |
3.39 |
| 11 |
McGuffin, Kathryn G (15)
|
VANOVA |
1:05.86 |
1:10.98 |
5.12 |
|
Coleman, Tess H (16)
|
VACCA |
1:00.75 |
NS |
- |
|
Kadela, Ann E (15)
|
VACCA |
1:02.66 |
NS |
- |
|
Lawrence, Peyton E (18)
|
VACCA |
57.27 |
NS |
- |