| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
King, Abigail (15)
|
AMCSC |
2:32.11 |
2:36.80 |
4.69 |
| 2 |
Sipula, Estella A (13)
|
AMWHAT |
2:40.56 |
2:39.68 |
-0.88 |
| 3 |
Race, Delaney M (13)
|
AMMMSA |
2:53.29 |
2:47.55 |
-5.74 |
| 4 |
Tursky, Addison G (14)
|
AMCSC |
2:39.01 |
2:58.42 |
19.41 |
| 5 |
Conwell, Natalie G (14)
|
AMWHAT |
2:55.15 |
2:58.75 |
3.60 |
| 6 |
White, Brooklyn R (14)
|
AMHAAC |
3:26.59 |
3:00.43 |
-26.16 |
| 7 |
Wukawitz, LaReyne E (13)
|
AMHAAC |
3:10.50 |
3:05.70 |
-4.80 |
| 8 |
Feinberg, Sivon R (13)
|
AMJCCS |
3:05.50 |
3:09.72 |
4.22 |
| 9 |
Greene, Emma M (14)
|
AM3RVS |
3:16.29 |
3:28.87 |
12.58 |
| 10 |
Roth, Ella S (14)
|
AMRXA |
3:41.74 |
3:42.27 |
0.53 |
| 11 |
Slotterback, Liesl A (13)
|
AMJCCS |
3:45.21 |
3:44.36 |
-0.85 |
| 12 |
Huntington, Brianna R (14)
|
AM3RVS |
3:47.57 |
4:03.37 |
15.80 |