| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ohtani, Haruki (10)
|
MDCAA |
44.90 |
39.11 |
-5.79 |
| 2 |
Holt, Dylan T (10)
|
MDFSC |
43.17 |
40.52 |
-2.65 |
| 3 |
Dolezel, Erik W (10)
|
MDCAA |
42.67 |
43.95 |
1.28 |
| 4 |
Hill, Wesley J (9)
|
VAHNVR |
48.41 |
46.09 |
-2.32 |
| 5 |
Jacobs, Lucas J (10)
|
MDMAC |
44.65 |
46.82 |
2.17 |
| 6 |
Suprun, Maximilian E (10)
|
MDCAA |
50.29 |
48.50 |
-1.79 |
| 7 |
Croxton, Alden R (10)
|
VAHNVR |
56.46 |
48.96 |
-7.50 |
| 8 |
Ballentine, Troy A (9)
|
MDMAC |
51.13 |
49.00 |
-2.13 |
| 9 |
Swafford, Edward P (9)
|
MDMAC |
1:00.09 |
51.63 |
-8.46 |
| 10 |
Hughes, Conner G (9)
|
VAWST |
49.68 |
51.64 |
1.96 |
| 11 |
De Los Reyes-Kundey, Aden A (10)
|
MDMAC |
50.63 |
54.56 |
3.93 |
| 12 |
Otten, Kellan P (10)
|
MDCAA |
1:11.11 |
54.59 |
-16.52 |
| 13 |
MacDougall, Tate D (9)
|
VARAYS |
1:00.92 |
57.27 |
-3.65 |
| 14 |
Yaselli, Kevin J (10)
|
MDMAC |
1:20.55 |
58.03 |
-22.52 |
| 15 |
Franz, Brice R (9)
|
VAWST |
1:39.12 |
1:02.02 |
-37.10 |
| 16 |
Sutherland, Reid A (9)
|
MDMAC |
1:09.03 |
1:02.35 |
-6.68 |
| 17 |
Midgette, Gus A (9)
|
MDMAC |
1:10.00 |
1:07.04 |
-2.96 |
| 18 |
Nardelli, Noah J (9)
|
VAWST |
1:25.12 |
1:07.44 |
-17.68 |
|
Voas, Elliot D (10)
|
MDMAC |
57.67 |
DQ |
- |
|
Parker, Declan P (9)
|
VARAYS |
53.41 |
NS |
- |