| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Merrill, Noah (10)
|
LEAYAA |
43.39 |
41.62 |
-1.77 |
| 2 |
Smith, Talan J (10)
|
DYP |
42.36 |
42.27 |
-0.09 |
| 3 |
Parkinson, Garrett (9)
|
OHLCCY |
42.85 |
44.44 |
1.59 |
| 4 |
Hart, Charles (10)
|
OHLCCY |
45.00 |
45.01 |
0.01 |
| 5 |
Brantley, Ben (10)
|
TUSC |
46.73 |
46.36 |
-0.37 |
| 6 |
Iskra, AJ (10)
|
LEGO |
48.25 |
46.54 |
-1.71 |
| 7 |
Cassidy, John (9)
|
OHLCCY |
45.00 |
47.07 |
2.07 |
| 8 |
Dechristofaro, Elliot J (10)
|
LEYYN |
50.35 |
50.75 |
0.40 |
| 9 |
Prelerson, Jacob J (9)
|
AMSVY |
54.82 |
52.77 |
-2.05 |
| 10 |
Dorenkott, Evan (10)
|
TUSC |
54.44 |
53.14 |
-1.30 |
| 11 |
Arabadzic, Marko (9)
|
OHLCWE |
52.51 |
54.75 |
2.24 |
| 12 |
Eisentraut, Tyler J (10)
|
LECFYN |
54.31 |
55.78 |
1.47 |
| 13 |
Cunningham, Kaden (10)
|
LEGYB |
58.21 |
57.50 |
-0.71 |
| 14 |
Turnbull, Alexander M (9)
|
LECFYN |
59.75 |
1:03.80 |
4.05 |
|
Smelko, Ayden P (9)
|
LEGO |
56.27 |
DQ |
- |
|
Cullivan, Casey (9)
|
OHLCCY |
54.68 |
NS |
- |