| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Alexander, Lexi J (11)
|
NCGFSC |
39.36 |
39.20 |
-0.16 |
| 1 |
Walker, LaiCie Mei R (11)
|
VADYST |
40.74 |
39.48 |
-1.26 |
| 1 |
Shelton, Julia E (12)
|
NCRACY |
|
44.75 |
- |
| 2 |
Girouard, Madelyn G (12)
|
NCRMY |
41.95 |
40.87 |
-1.08 |
| 2 |
Goodnight, Caroline R (11)
|
NCACCH |
52.56 |
46.71 |
-5.85 |
| 3 |
Preslar, Lena A (12)
|
NCRACY |
42.74 |
41.91 |
-0.83 |
| 3 |
Lineburger, Gracie B (12)
|
NCRACY |
59.34 |
47.04 |
-12.30 |
| 4 |
Brewer, Rylee G (11)
|
NCRMY |
42.71 |
42.13 |
-0.58 |
| 4 |
Honeycutt, Caroline H (12)
|
NCVACB |
47.84 |
47.38 |
-0.46 |
| 5 |
Tanner, Olivia M (11)
|
NCACCH |
50.49 |
50.16 |
-0.33 |
| 6 |
Stokes, Elizabeth A (11)
|
NCYBAC |
53.92 |
50.46 |
-3.46 |
| 7 |
Jernigan, Jordyn M (11)
|
NCRACY |
50.34 |
51.02 |
0.68 |
| 8 |
Wohlschlegel, Abigail R (11)
|
NCGFSC |
|
1:30.91 |
- |
|
Mountain, Erin M (12)
|
NCGFSC |
|
DQ |
- |