| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Zhou, Felix (11)
|
NCNCAC |
41.10 |
40.74 |
-0.36 |
| 1 |
Whitley, Luke E (11)
|
NCNCAC |
43.65 |
42.72 |
-0.93 |
| 2 |
Carneiro, Brennen T (11)
|
NCUN |
41.69 |
41.63 |
-0.06 |
| 2 |
Sheldon, Warner G (11)
|
NCNCAC |
43.96 |
44.43 |
0.47 |
| 3 |
McDougal, Cavin Z (12)
|
NCRACY |
49.17 |
47.08 |
-2.09 |
| 4 |
Hunter, Levi G (12)
|
NCNCAC |
55.93 |
48.07 |
-7.86 |
| 5 |
Pease, Bradon A (12)
|
NCNCAC |
|
49.48 |
- |
| 6 |
Zhang, Shawn (11)
|
NCNCAC |
55.00 |
49.95 |
-5.05 |
| 7 |
Rowe, Silas D (12)
|
NCHAC |
50.39 |
49.97 |
-0.42 |
| 8 |
Radvansky, James (12)
|
NCNCAC |
|
50.10 |
- |
| 9 |
Bassett, Raiden H (12)
|
VAHAVA |
49.64 |
50.65 |
1.01 |
| 10 |
Bassett, Remi J (12)
|
VAHAVA |
52.90 |
53.42 |
0.52 |
| 11 |
Hathaway, Caleb A (11)
|
NCNCAC |
52.00 |
57.65 |
5.65 |
| 12 |
Smith, Cole J (11)
|
NCRACY |
1:02.58 |
1:01.37 |
-1.21 |
|
Vermunt, Gunner L (11)
|
NCNCAC |
|
DQ |
- |
|
Fabrizio, Maximilian R (11)
|
NCNCAC |
|
DQ |
- |
|
Porter, Noah W (12)
|
NCNCAC |
41.41 |
NS |
- |