| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mostek, Anya H (17)
|
MAPAY |
57.96 |
57.55 |
-0.41 |
| 2 |
Johnson, Natalie M (15)
|
MAWEST |
1:01.82 |
57.59 |
-4.23 |
| 3 |
Fenn, Victoria A (16)
|
MAPFY |
1:00.03 |
58.32 |
-1.71 |
| 4 |
Zelinske, Lauren N (16)
|
MAPAY |
1:00.76 |
59.41 |
-1.35 |
| 5 |
Testa, Madison N (15)
|
MAWEST |
59.86 |
1:00.06 |
0.20 |
| 6 |
Humphrey, Delaney G (17)
|
MAGSVY |
1:03.87 |
1:01.37 |
-2.50 |
| 7 |
Daisey, Aya M (17)
|
MASFY |
1:01.74 |
1:01.38 |
-0.36 |
| 8 |
Rydbom, Layne (15)
|
MACC |
1:03.86 |
1:02.48 |
-1.38 |
| 9 |
Pappas, Penelope E (17)
|
MAPAY |
1:01.34 |
1:02.96 |
1.62 |
| 10 |
Keller, Janell (16)
|
MAUPY |
1:04.20 |
1:03.93 |
-0.27 |
| 11 |
Schweigert, Caroline E (15)
|
MALANY |
1:07.35 |
1:04.09 |
-3.26 |
| 12 |
Wynn, Rozlyn L (15)
|
MAWEST |
1:07.53 |
1:04.28 |
-3.25 |
| 13 |
McKenrick, Allysandra G (17)
|
MAPAY |
1:04.51 |
1:04.94 |
0.43 |
| 14 |
Calder, Ella C (16)
|
MAYY |
1:05.52 |
1:05.10 |
-0.42 |
| 15 |
Fung, Joyce (18)
|
MAPAY |
1:06.17 |
1:07.80 |
1.63 |
| 16 |
Hunt, Kiera (17)
|
MALYM |
1:04.49 |
1:07.93 |
3.44 |
| 17 |
Stroble, Emily (17)
|
MACC |
1:05.73 |
1:08.44 |
2.71 |
| 18 |
Sponsler, Te'ah L (17)
|
MANDCY |
1:07.53 |
1:08.65 |
1.12 |
| 18 |
Froman, Emily J (17)
|
MAYY |
1:06.32 |
1:08.65 |
2.33 |
|
Eastman, Natalie A (17)
|
MAPFY |
1:04.29 |
NS |
- |
|
Ruben, Taleah A (17)
|
MANDCY |
1:03.13 |
NS |
- |